Journal: Cell Death & Disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: a , e shows the expression of SPARCL1 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group and pSPgRNA is the blank control for SPARCL1 activation. NC was the negative control for SPARCL1 siRNA interference. b – d are grayscale scans of the proteins shown in a . f – h are grayscale scans of the proteins shown in e . i , k show Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j , l shows the quantification of myotubes according to the Desmin staining of I and K. The scale bar in I and K is 100 μm; the green fluorescent signal is Desmin, while the blue fluorescent signal is the nucleus. ** P < 0.01 were considered as significant

Article Snippet: Different fragments were cloned into the vector of pSPgRNA vector (Addgene, Middlesex, UK), and the pSPgRNA vector was cleaved with a BbsI restriction endonuclease and ligated to the annealed fragment and named as: pSPgRNA-S-1, pSPgRNA-S-2, pSPgRNA-S-3, pSPgRNA-B-1, pSPgRNA-B-2, and pSPgRNA-B-3.

Techniques: Expressing, Activation Assay, Control, Negative Control, Staining

Journal: Cell Death & Disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: a , e show the expression of BMP7 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-B-3 was the BMP7 activation group, pSPgRNA was the blank control for BMP7 activation. NC was a negative control for BMP7 siRNA interference. b – d shows greyscale scans of the proteins in A. f – h shows greyscale scans of the proteins in e . i , k shows Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j , l shows quantification of myotubes according to Desmin staining in i , k , respectively. The SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β scale bar in i , k is 100 μm, and the green fluorescent signal is Desmin, while the blue fluorescent signal is the nucleus. ** P values < 0.01 and * P values < 0.05 were considered as significant

Article Snippet: Different fragments were cloned into the vector of pSPgRNA vector (Addgene, Middlesex, UK), and the pSPgRNA vector was cleaved with a BbsI restriction endonuclease and ligated to the annealed fragment and named as: pSPgRNA-S-1, pSPgRNA-S-2, pSPgRNA-S-3, pSPgRNA-B-1, pSPgRNA-B-2, and pSPgRNA-B-3.

Techniques: Expressing, Activation Assay, Control, Negative Control, Staining, Cell Differentiation

Journal: Cell Death & Disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: a , d shows the protein expression of BMP7 regulated by SPARCL1 activation and inhibition, respectively. C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. b , c are greyscale scans of SPARCL1 and BMP7 proteins in A. e , f are grayscale scans of SPARCL1 and BMP7 proteins in D. i and p shows changes in the expression of BMP/TGF-β-associated proteins when SPARCL1 was activated and inhibited, respectively, and C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. j – o are greyscale scans of proteins in I . q – v are greyscale scans of proteins in P . ** P values < 0.01 were considered as significant

Article Snippet: Different fragments were cloned into the vector of pSPgRNA vector (Addgene, Middlesex, UK), and the pSPgRNA vector was cleaved with a BbsI restriction endonuclease and ligated to the annealed fragment and named as: pSPgRNA-S-1, pSPgRNA-S-2, pSPgRNA-S-3, pSPgRNA-B-1, pSPgRNA-B-2, and pSPgRNA-B-3.

Techniques: Expressing, Activation Assay, Inhibition, Control, Negative Control

Journal: Cell Death & Disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: a shows the expression of proteins related to TGF-β signaling pathway. b – h are greyscale scans of proteins in A. h shows Desmin expression in C2C12 cells when SPARCL1 was activated and BMP7 was inhibited at 72 h. i is the corresponding immunofluorescence blot of Desmin protein in A. In a , i , pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β blank control for SPARCL1 activation. SiRNA-B is the BMP7 interference group. NC is a negative control for BMP7 siRNA interference. The scale bar in I is 100 μm, green fluorescent signal is Desmin, and blue fluorescent signal is the nucleus. ** P values < 0.01 and * P values < 0.05 were considered as significant

Article Snippet: Different fragments were cloned into the vector of pSPgRNA vector (Addgene, Middlesex, UK), and the pSPgRNA vector was cleaved with a BbsI restriction endonuclease and ligated to the annealed fragment and named as: pSPgRNA-S-1, pSPgRNA-S-2, pSPgRNA-S-3, pSPgRNA-B-1, pSPgRNA-B-2, and pSPgRNA-B-3.

Techniques: Expressing, Immunofluorescence, Activation Assay, Cell Differentiation, Control, Negative Control